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DNA extraction Media Prime buffer: 0.1 M EDTA–KOH pH 7.5 and 10 mM DTT Zymolyase buffer: 20 mM K-phosphate pH 7.2, 1.2 M sorbitol, 0.4 mg ml −1 Zymolyase 20T (amsbio, USbiological) and 100 μg ml −1 RNAse A Homemade P1: 50 mM Tris–HCl pH 8.0, 10 mM EDTA and 100 μg ml −1 RNAse A Homemade P2: 200 mM NaOH and 1% SDS Miniprep for 200-ml yeast cultures Cell pellets were thawed at RT, resuspended in 4 ml prime buffer and incubated at 30 °C for 15 min