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367 Although the Cu I specificity of this switch has not yet been unequivocally evaluated, residues in the second coordination shell are conserved, as are the neighboring genes found in all three Cu I -specific clusters (a P 1B-1 CopA efflux pump and a CopZ metallochaperone)

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Compounds at 500 mM in DMSO stocks were dispensed into selected crystal drops as singletons using an Echo 550 liquid handler to give a final concentration of 50 mM (10% DMSO final concentration)