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[DOI] [PubMed] [Google Scholar] 221.Gaillard P.J., Appeldoorn C.C., Dorland R., van Kregten J., Manca F., Vugts D.J., Windhorst B., van Dongen G.A., de Vries H.E., Maussang D., et al
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To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress ® II One Step Cloning Kit
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