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Cells were incubated at 37 °C (5% CO 2 ) for 6 days and the effects of drugs on organoids viability were determined using Resazurin cell viability assays

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Furthermore, NAC has been shown to influence redox-sensitive cell-signaling and transcription pathways, such as NF- κ B (which regulates pro-inflammatory genes), and the p38, ERK1/2, SAPK/JNK, c-Jun, and c-Fos pathways, among others, in a wide variety of different systems [139, 143]