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Furthermore, structure-guided site-directed mutagenesis and alanine scanning and saturation mutagenesis of Lb TDC were performed on residues around the substrate binding sites and those required for conformational stability to elucidate the function of key residues involved in the catalytic mechanism and to promote the potential applications of Lb TDC in tyramine synthesis, food safety, and pharmacology

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The transduced cells were then selected with puromycin (1 μg/mL) for 5–7 days to establish stable lines