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Once cellular antioxidant defenses are depleted, hyperglycemia drives excessive mitochondrial ROS production and promotes protein glycation, leading to the formation of advanced glycation end products (AGEs)

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Agarose beads were then washed three times in IP buffer followed by three times wash in TAE buffer and incubated for 5 minutes at 95 °C in NuPAGE LDS Sample Buffer (Invitrogen)